do you mean earlier whne coot was connected to phenix during the refinement? If 
you load the pdb and the mtz directly into coot, you have to load the cif file 
beofre starting the real space refinement in coot.


Dr. Matthias Barone

AG Kuehne, Rational Drug Design

Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP)
Robert-Rössle-Strasse 10
13125 Berlin

Germany
Phone: +49 (0)30 94793-284

________________________________
From: CCP4 bulletin board <[email protected]> on behalf of Muhammad Bashir 
Khan <[email protected]>
Sent: Friday, May 1, 2020 2:25:56 AM
To: [email protected]
Subject: Re: [ccp4bb] Ligand Refinement in coot

ReadySet

On Thu, Apr 30, 2020 at 6:23 PM Sharan Karade 
<[email protected]<mailto:[email protected]>> wrote:
Hi,
 I think, you can generate new restrain file for your ligand and load before 
you do refine in COOT for next time. I have query that, how did you generate 
restrain file for ligand (elbow or readyset in phenix)?.




On Thu, Apr 30, 2020 at 5:06 PM Muhammad Bashir Khan 
<[email protected]<mailto:[email protected]>> wrote:
Hi Folks;

I want to ask about the ligand refinement in coot. Earlier I was able to refine 
the ligand in the coot using real space refine zone but now by some reason when 
I want to refine the ligand in coot it asks me "Refinement setup failure failed 
to find restrained for XYZ". Do not know what happen to the restrained file

All your suggestion will be highly appreciated

Bashir

--
------------------------------------------------------
Muhammad Bashir Khan, Ph.D.
Research Associate
Department of Biochemistry
Medical Science Bldg.
Lab 3-27
University of Alberta
Edmonton AB, T6G 2H7

Phone: 780-492-4577-
e-mail: [email protected]<mailto:[email protected]>

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--
Sharan Karade
Postdoc-fellow
IBBR-UMD, 9600 Gudelsky Dr,
Rockville
Maryland 20850




--
------------------------------------------------------
Muhammad Bashir Khan, Ph.D.
Research Associate
Department of Biochemistry
Medical Science Bldg.
Lab 3-27
University of Alberta
Edmonton AB, T6G 2H7

Phone: 780-492-4577-
e-mail: [email protected]<mailto:[email protected]>

________________________________

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