Dear Murpholino,
a portuguese company called NZYtech has a collection of fusion tag pHTP
expression vectors
(https://www.nzytech.com/wp-content/uploads/2019/04/nzyeasy-cloning-expression-system_userguide_v1901.pdf),
some of them designed for increased protein's solubilization.
We have a recent example of using a Ketosteroid isomerase (KSI) tag that
highly increased solubility and crystallizability of one of our tagets
(a kinase). No news on phasing and phase extension since crystals were
not screened yet due to this long lock-down.
HTH, best,
Jose
Subject Fusion proteins to solubilize your protein
From Murpholino Peligro
Date Tue 19:44
I was wondering how many proteins that help to crystallize the protein
of interest are out there...
and how effective is the phase extension method (i.e. use MR to get the
structure of the fusion protein into its density and the density of the
protein of interest...)
Is there a way to get this data from the PDB?
Here is my list so far:
- MBP
- GFP
- TRX
- Lysozyme
- GST
Thanks
--
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