This reminds me of something that we crystallized a few years back. It indexed as I222 (or I2(1)2(1)2(1)) and the cell was quite small; too small for the protein of interest. Almost certainly a contaminant, but it didn’t show up in Contaminer. Diffracted like gangbusters, but we never figured out what it is. Very annoying. Unfortunately I can’t tell you cell constants, because those data are locked in my office and my institution is locked down...
> On 22 May 2020, at 6:08 AM, Demou, Maria <maria.demou...@ucl.ac.uk> wrote: > > Dear all, > I have a question that may have a straight forward answer, and was wondering > if this is a common issue. We have a protein crystallised in I222 space > group. This is CRP, but the monomer/pentamer is not predicted to fit in this > space group. Is there a possibility of the lipid cubic phase being > crystallised on it's own, or is there any other obvious reason? > > Thank you, > Maria > > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > <https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1> __________________________ Patrick J. Loll, PhD Professor of Biochemistry & Molecular Biology Drexel University College of Medicine Room 10-102 New College Building 245 N. 15th St. Philadelphia, PA 19102-1192 USA (215) 762-7706 pj...@drexel.edu <mailto:pj...@drexel.edu> pjl...@gmail.com ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
