Hey Suchi, you could firstly increase the binning to factor to 4-5. Also, it would help to shrink the mask radius. Most definitely keep the resolution limited to 8-10 Å!
Cheers Schara Dr. Schara Safarian Max Planck Institut für Biophysik Abteilung für Molekulare Membranbiologie Max Planck Institute of Biophysics Department of Molecular Membrane Biology Max-von-Laue-Straße 3 60438 Frankfurt am Main Deutschland/Germany Twitter <https://twitter.com/SafarianSchara> Google Scholar <https://scholar.google.com/citations?hl=de&user=SjePlggAAAAJ> > Am 01.06.2020 um 07:18 schrieb Suchi Goel <suchig...@gmail.com>: > > Hi Everyone, > > I am new to this list. Have some problem of background in 2D classification, > that I can't get rid of for any parameter of regularization (1.4-2.0) or > resolution limit (-1, no limit and 7-12 A limit). > Attached files are the output classes in Binning 2 and 1 respectively. > Could some one help who has this experience. > > Best Regards, > Suchi Goel > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > <https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1><Screen Shot > 2020-06-01 at 14.07.37.png><Screen Shot 2020-06-01 at 14.06.54.png> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
