Hello,

I'm refining protein structure in Refmac and I have NCS greater than 4 ( I've 8 
identical subunits in the asymmetric unit). 

In the "Refinement parameters" section in Refmac, how can I pick Rfree set 
based on thin resolution shells rather than in the normal random method?

I've read about the program "dataman" from the usf suite which could help with 
this but found it very complicated. 

Do you have any advice to solve this? or would you recommend any other program? 


Thank you in advance 

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