Hi all I'm designing genes for expression in M. smegmatis (safe host for Mtb proteins), but its not possible (or advisable due to the GC content) to optimise for mycobacterial expression.
Would anyone with experience be able to tell me if its fine to stick with the E. coli codon optimisation, or if there is an advantage going with b. subtilis (or another G+ organism that is in Thermo's list). Best wishes Matthew. ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
