Dear All, many thanks to you all for your suggestions and help! I learned about some interesting small companies producing proteins and had other useful suggestions, some already shared with the list. Another example of the wonderful CCP4bb community. In the end it looks like we will go for His-tagged GFP, because it will have specific advantages for the experiments, and someone can produce it locally here for what will be a good price (don't have an exact quote yet and won't advertise the outfit unless they desire this).
Lesser known (at least not previously known to me) companies that were recommended are: http://www.molirom.com/ <http://www.molirom.com/> https://www.37cbio.com/ <https://www.37cbio.com/> https://www.novusbio.com/ <https://www.novusbio.com/> Acrobiosystems.com <http://acrobiosystems.com/> (I'm think I can be sure that they are happy to be named) Best wishes, Mark Mark J van Raaij Dpto de Estructura de Macromoleculas Centro Nacional de Biotecnologia - CSIC calle Darwin 3 E-28049 Madrid, Spain tel. (+34) 91 585 4616 Section Editor Acta Crystallographica F https://journals.iucr.org/f/ > On 2 Jul 2020, at 11:50, Tristan Croll <ti...@cam.ac.uk> wrote: > > Thermo Fisher sells His-tagged GFP quite cheaply. > https://www.thermofisher.com/order/catalog/product/A42611#/A42611 > <https://www.thermofisher.com/order/catalog/product/A42611#/A42611> > Tristan > > > > > On 2020-07-02 10:31, Mark J van Raaij wrote: > >> Dear All, >> >> Wondering if anyone knows of an economical commercial source of a soluble >> His-tagged protein. In principle any His-tagged protein would suffice, >> because it's for testing coupling to a surface via the His-tag. Asking for >> an international collaborator, who don't have access/expertise of a >> molecular biology lab (except us :-). >> We can and will make the His-tagged proteins of interest for them, but if >> there is an economical and reliable commercial source, it might be worth >> using that for initial tests. >> >> Best wishes, >> >> Mark >> >> Mark J van Raaij >> Dpto de Estructura de Macromoleculas >> Centro Nacional de Biotecnologia - CSIC >> calle Darwin 3 >> E-28049 Madrid, Spain >> tel. (+34) 91 585 4616 >> Section Editor Acta Crystallographica F >> https://journals.iucr.org/f/ <https://journals.iucr.org/f/> >> >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 >> <https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1> > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > <https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
