Keep the protein concentration low during purification steps along with using
other anti-aggregation agent/s. Make sure that the pH at which you are
purifying is not close to the pI of the protein. Until completely purified, all
purification steps should be performed in a cold room if it is a soluble
protein.
Smita
On Sunday, July 19, 2020, 04:28:45 AM CDT, Sorin Draga
<[email protected]> wrote:
I am not sure what you mean by polymer formation. Presuming that you have
optimized your protein concentration, pH and salt concentration, you could try
arginine as an anti-aggregation agent in your purification (I presume you do
FPLC). Have a look at chaotropic agents used in protein purification, The
answer is generally dependent on the protein/proteins you are trying to purify
and is not necessarily straightforward.
Kinds regards
On Sun, Jul 19, 2020 at 12:08 PM 张士军 <[email protected]> wrote:
Dear all:
Any ideas to decrease protein polymer formation? my protein was easy to form
oligomers and precipitation when do purification,I have tried add glycerol and
DTT,both didn't work. Does anyone has experience to avoid it happening. Thanks!
Best Regards
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