<div style="font-family:Arial, Helvetica, sans-serif; font-size:12px;">Dear Samer,<br> <br> What king of cryo-protectant did you use ?<br> <br> Such a bent density, with that size, looks like an ethylene-diol.<br> <br> Best regards,<br> <br> Nils Marechal</div>
<div class="gl_quote" style="margin-top: 20px; padding-top: 5px;">De : "samer halabi" <[email protected]><br> A : [email protected]<br> Envoyé: lundi 20 Juillet 2020 18:17<br> Objet : [ccp4bb] electron density close Histidine side chain<br> <div class="gl_quoted"> <div class="yahoo-style-wrap" style="font-family:times new roman, new york, times, serif;font-size:16px;"> <div> <div data-setdir="false" dir="ltr"><span style="color:rgb(51, 51, 51); font-family:monospace; white-space:nowrap">Hello all,</span><br style="color: rgb(51, 51, 51); font-family: monospace;"> <span style="color:rgb(51, 51, 51); font-family:monospace">I have few blobs in an MHC II structure I am working on, especially opposite to Histidine as in the accompanying screenshot, that I am confused about.</span><br style="color: rgb(51, 51, 51); font-family: monospace;"> <br style="color: rgb(51, 51, 51); font-family: monospace;"> <span style="color:rgb(51, 51, 51); font-family:monospace">In the crystal conditions, I have Tris, Imidazole, Acetate, PEG and Glycerol.</span><br style="color: rgb(51, 51, 51); font-family: monospace;"> <span style="color:rgb(51, 51, 51); font-family:monospace">Whatever ligand I am fitting in I am getting a clash (overlap -1.029), which makes me think whether there is a covalent bond forming between Histidine and other molecule. Perhaps by oxidation.</span><br style="color: rgb(51, 51, 51); font-family: monospace;"> <br style="color: rgb(51, 51, 51); font-family: monospace;"> <span style="color:rgb(51, 51, 51); font-family:monospace">I would greatly appreciate if you can advice me about it, whether there is some kind of ligand I can try to fit and if this is something that occurs in some structures.</span><br style="color: rgb(51, 51, 51); font-family: monospace;"> <span style="color:rgb(51, 51, 51); font-family:monospace; white-space:nowrap">Thank you.</span><br style="color: rgb(51, 51, 51); font-family: monospace;"> <span style="color:rgb(51, 51, 51); font-family:monospace; white-space:nowrap">Best regards,</span><br style="color: rgb(51, 51, 51); font-family: monospace;"> <span style="color:rgb(51, 51, 51); font-family:monospace">Samer</span></div> </div> </div> <hr> <p align="center">To unsubscribe from the CCP4BB list, click the following link:<br> <a href="https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1"; target="_blank">https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1</a></p> </div> <div class="gl_quoted"> </div> </div> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
