Dear All, I am new to negative staining (and hopefully cryoEM soon) and wanted to ask for your advice before I proceed. I am trying to visualize DNA, both circular and linear (2200b.p.), I am using 2% Uranyl Acetate and regular carbon grids. I’ve been getting unclear images under TEM, some strings which I doubt are what I am looking for. I would highly appreciate any advice, for example what are the DNA concentrations I should work with? What dye? How long should I let the DNA stay on the grid prior to staining? Is there an optimal size for a plasmid ? Thank you, Meytal
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