Hi, All, I am new to MicroED (microcrystal electron diffraction). I know that X-ray crystallography has phase problem, and I think MicroED has phase problem too (it is diffraction of electron instead of x-ray). However, when I read the Wikipedia, I could not understand the following description of MicroED: One of the main difficulties in X-ray crystallography is determining phases in the diffraction pattern. Because of the complexity of X-ray lenses, it is difficult to form an image of the crystal being diffracted, and hence phase information is lost. Fortunately, electron microscopes can resolve atomic structure in real space and the crystallographic structure factor phase information can be experimentally determined from an image's Fourier transform. The Fourier transform of an atomic resolution image is similar, but different, to a diffraction pattern—with reciprocal lattice spots reflecting the symmetry and spacing of a crystal.
Does the above description mean that MicroED, or more broadly electron crystallogaphy does NOT suffer from phase problem? How about single particle cryo electron microscopy, it should NOT have phase problem, right? Thanks for any input in it. Best, Alex ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
