Hello Abhik

In coot use: Calculate>Merge Molecules to append one structure to another.  
Coot will change the chain labels for you, which might work out OK in this case.

Alternatively, I've done a lot of this in my time:
Make a copy of the reference PDB file
Open it in a simple text edit (emacs, vi etc)
Go to the end, remove the PDB 'END' statement
(Many programs stop reading a PDB file when they see an END)
Append the second, superimposed file.
For most chain labels you can also change them in a text editor but 'C' 'O' and 
'N' will cause you trouble.  You can do it easily in Coot.
This would take no more than 15 seconds in emacs.

>From the command line:
grep -v 'END' reference.pdb > reference2.pdb
egrep '^ATOM|^HETATM' superimposed.pdb >> reference2.pdb
(text editor to change chain labels, if you care, but there are ways to do that 
from the command line too)

Cheers
Phil Jeffrey
Princeton

________________________________
From: CCP4 bulletin board <[email protected]> on behalf of Abhik 
Mukhopadhyay <[email protected]>
Sent: Monday, September 14, 2020 4:55 PM
To: [email protected] <[email protected]>
Subject: [ccp4bb] How to save two superposed protein structures in PDB format

Hi,
How can I save two superposed protein structures in  PDB format? Is there any 
way I can do this in coot or pymol? There is only one chain in those two PDB 
structures.

Thanks in advance,
Abhik



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