Dear Christian, Kleywegt (1996; "Use of Non-crystallographic Symmetry in Protein Structure Refinement." Acta Cryst D52, 842-857. DOI: 10.1107/S0907444995016477) reported that NCS-related molecules tend to be similar but differences in ligand binding do sometimes occur (cited Sevcik et al. 1996 in that paper).
We saw a similar difference (coenzyme absent/present; co-crystallized) between 2 biologically monomeric NCS-related protein molecules apparently due to crystal contact differences (Sidhu et al. 2011; "Structure of a highly NADP+-specific isocitrate dehydrogenase." Acta Cryst. D67, 856-869. DOI: 10.1107/S0907444911028575). Best wishes, Navdeep --- On 27.10.20 11:19, Christian GALICIA wrote: > Hello, > In our structure only one chain in a crystallographic trimer > (non-biological) shows a ligand bound to it (with clear density). There > doesn't seem to be any channels (or lack of them) favoring that specific > site. Can the community give your opinion on whether this can make the > presence of the ligand or its biological role questionable, and give any > examples of similar cases you might be aware of. Thank you. > -- > *Christian Galicia* > Post Doctoral Scientist > E-mail: cgali...@vub.be <mailto:cgali...@vub.be> > > > > > ------------------------------------------------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
