Dear Silvia, While high Bs can be a sign that parts of your model are incorrect (and therefore some careful manual rebuilding may be required), for things like poorly-ordered surface loops, they can simply be truth. A point worth remembering for newbies: your goal is to produce the model that best reflects the data, which seems obvious, but that means don't try to model what you can't see (e.g. seriously disordered surface loops) and don't worry if bits where the density was marginal have high Bs. Best, Phoebe
On 1/4/21, 8:39 AM, "CCP4 bulletin board on behalf of Silvia Napolitano" <[email protected] on behalf of [email protected]> wrote: Dear CCP4 Community, I am currently working on the structure of a monomeric protein of 23KDa. The protein is generally quite "loopy" and I think I am mid-way refinement. At the moment I am struggling, among other things, with an extremely high B-factor and I'm not sure how to lower it. Do you have any suggestions on how I can proceed to obtain a lower average B-factor? (I am sorry if the question is rather naive, but I have worked very little on X-ray structures so far) I attached a screenshot of the polygon obtained from the last refinement (the geometry now should be a bit improved, I am running a new job at the moment). I will be happy to provide further files if needed. Many thanks in advance for your precious help! I wish you a nice start to the new year. Best Silvia ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/ ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
