Hi all,
I am struggling with the MR of a homo-dimer using Phaser. Matt_coeff strongly
suggests the presence of 4 mol per asym unit (space group P6222). The results
after a search of 4 mol per asymmetric unit of my monomer are the following:
** SINGLE solution
** Solution written to SOL file: phaser_3f6v_A1_MOLREP.sol
** Solution written to PDB file: phaser_3f6v_A1_MOLREP.1.pdb
** Solution written to MTZ file: phaser_3f6v_A1_MOLREP.1.mtz
Solution annotation (history):
SOLU SET RFZ=3.9 TFZ=8.5 PAK=3 LLG=65 TFZ==10.0 RFZ=2.6 TFZ=17.0 PAK=3
LLG=326 TFZ==29.8 (& TFZ==22.5 & TFZ==19.7)
LLG+=(326 & 529 & 593) LLG=795 TFZ==5.4 PAK=5 LLG=795 TFZ==5.4 PAK=5
LLG=795 TFZ==5.4
SOLU SPAC P 62 2 2
SOLU 6DIM ENSE autoMR EULER 125.3 60.8 300.2 FRAC 0.17 -0.13 0.08 BFAC
-9.34 #TFZ==10.0
SOLU 6DIM ENSE autoMR EULER 305.4 60.8 300.2 FRAC 0.27 -0.16 0.08 BFAC
-5.29 #TFZ==29.8
SOLU 6DIM ENSE autoMR EULER 294.7 119.1 120.3 FRAC 0.45 0.01 0.26 BFAC
0.34 #TFZ==22.5
SOLU 6DIM ENSE autoMR EULER 305.5 61.6 300.1 FRAC 0.11 -0.16 0.08 BFAC
29.19 #TFZ==5.4
SOLU ENSEMBLE autoMR VRMS DELTA -0.2463 #RMSD 0.93 #VRMS 0.79
It seems (also looking at the maps) that it correctly places three monomers out
of four. How can I use this information to improve the search of the fourth
monomer using the same template model?
Thanks in advance for your help,
Best regards
Luca Mazzei
Luca Mazzei - PhD
Laboratory of Bioinorganic Chemistry
Department of Pharmacy and Biotechnology (FaBiT)
Alma Mater Studiorum - University of Bologna
Viale Giuseppe Fanin, 40 - 40127, Bologna - Italy
Tel: +39 0512096235
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