On Tuesday, 12 January 2021 15:22:18 PST rohit kumar wrote:
> Dear all,
>
> I am trying to solve a data with 3 A resolution, however data quality is
> very bad and mathews coffi. suggest two molecules per ASU but It always
> gives one molecule in AU after phaser with the TFZ and RFZ score are 4.5
> and 3.5 respectively with LLG gain 121.
> And when I used that model for Refmac the final R/Rfee is 23/30 and with
> satisfactory Ramachandran statistics as well as electron density and model
> in agreement with each other in coot. Does It mean that I have
> correct solution?
Sounds promising.
The other to check is that the model you have exhibits a set of lattice
contacts sufficient to form a believable crystal packing. If the
symmetry-related
molecules do not touch each other then there must be some component
missing from your model.
cheers,
Ethan
--
Ethan A Merritt
Biomolecular Structure Center, K-428 Health Sciences Bldg
MS 357742, University of Washington, Seattle 98195-7742
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