Sorry for the non-CCP4 question… I would like ask your advice on designing SUMO fusion proteins. Specifically, is it recommended to include a linker between the C-terminus of SUMO (-QIGG) and the N-terminus of the target protein? Figure 3 of Guerrero et al. (https://pubmed.ncbi.nlm.nih.gov/26297996/) suggests that including a linker of SGG or SGGG greatly improves cleavage efficiency by Ulp1. However, the often-stated advantage of SUMO is that, in the absence of a linker, the target protein contains no extra residues after cleavage.
I’ll also take any advice on your favorite N-terminal His tag for SUMO fusions. I am considering MGSSHHHHHHG, based on two Acta F papers (https://pubmed.ncbi.nlm.nih.gov/22949189/, https://pubmed.ncbi.nlm.nih.gov/18391421/), or perhaps MGSSHHHHHHSSG, which is from pET14b. Thanks, Jack -- John J. Tanner Professor of Biochemistry and Chemistry Associate Chair of Biochemistry Department of Biochemistry University of Missouri 117 Schweitzer Hall 503 S College Avenue Columbia, MO 65211 Phone: 573-884-1280 Email: tanne...@missouri.edu<mailto:tanne...@missouri.edu> https://cafnrfaculty.missouri.edu/tannerlab/ Lab: Schlundt Annex rooms 3,6,9, 203B, 203C Office: Schlundt Annex 203A ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
