Yes but without the methyl groups on the sugars. I scavenged a GTA capped RNA from a PDB - 5wfh - which draws the link from the GTA to the 5’-phosphate of the RNA, but when you regularise them in coot, it breaks the connecting bond.
However after just starting to reply to you I realised that the GTA is marked as HETATM, so I just edited it to ATOM in the PDB and the wonderful coot minimiser now bonds everything perfectly. Thanks all who responded. -------------------------------------------------------------------------------- Laurence H. Pearl PhD FRS FMedSci Professor of Structural Biology Genome Damage and Stability Centre School of Life Sciences, University of Sussex Brighton, BN1 9RQ, UK +44-(0)1273 678349 -------------------------------------------------------------------------------- "Live Modestly and do Serious Things .. " - Dorothy Crowfoot Hodgkin -------------------------------------------------------------------------------- On 17 May 2021, at 17:13, Paul Emsley <[email protected]<mailto:[email protected]>> wrote: On 17/05/2021 17:08, Laurence Pearl wrote: Can anyone tell me where to find a model dictionary entry for COOT and/or phenix for the 7meG-pp residue that forms the 5’ cap on a messenger RNA ? I need to be able to bond it to the 5’-end of an RNA model and bond it to the existing 5’-phosphate of the first ribonucleotide. Do you mean the orange/yellow entity here? Paul <Screen Shot 2021-05-17 at 17.12.20.png> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
