Dear CCP4bb, I have a partly specific question, relating to multidomain enzymes.
We have a nice hi-res structure of an enzyme, which comprises domain A (non-catalytic) and domain B (enzymatic) Through the magic of unstimulated in-tray proteolysis, there are one and a half copies in the ASU, one full AB and one A half The second (proteolysed) domain A is jammed in active site B of the full-length AB copy. The contacts look glorious and specific but we don't have enough sequence variation among homologues to say if interfaces are conserved. A full-length AB protein would never inhibit another because the AB domain arrangement would cause a steric clash. So my question is - "do you know of any enzyme where it gets proteolysed to generate a fragment that goes on to inhibit itself?". I'd prefer answers that are about fragments/complexes rather than say a zymogen state. Hope that makes sense Be great if you can tell me of any - not the easiest thing to search for! Thanks in advance Andy ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
