Hi Dhiraj - In addition to the other good suggestions, you may also wish to try codon optimization and different secretion signal peptides if your target protein is secreted.
Hope that helps. Cheers, Jared On Thu, Jun 24, 2021 at 4:42 PM Srivastava, Dhiraj < [email protected]> wrote: > Hi all > I am trying to express my protein in HEK293 cells for > crystallization purpose but getting poor expression. I am using cmv > promoter. Which promoter/vector people use to get good expression in > eukaryotic expression system? > > Thank you > > Dhiraj > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
