Dear Colleagues

You are cordially invited to attend the virtual CBMS Lecture Series .....


An outlook on cryo-electron microscopy for structural and cellular biology



Raimond Ravelli

Associate Professor

Institute of Nanoscopy (IoN), M4I,

Fac. Health, Medicine and Life Sciences

Maastricht University



July 14, 2021, 13:30 (EDT)



Register in advance for this meeting:
https://bnl.zoomgov.com/meeting/register/vJIsdOCopzouHUg5FoLj2CPuhFjhAZo8yis

Abstract

Cryo-EM has become a powerful tool for structural and cellular biologists to 
study the structure-function relationships of their biomolecules of interest. 
Recent development of TEM hardware has led to atomic resolution for single 
particle cryo-EM, and near-atomic resolution for macromolecular complexes 
within cells. Most of the current successes have been reached using 
conventional phase contrast cryo-TEM. In this talk, I will discuss current 
limitations in cryo-EM such as sample preparation and detectors and show some 
of the developments therein. Could it become possible to solve a structure, as 
small as hen egg white lysozyme, by single particle cryo-EM? We will review 
alternative, exotic cryo-EM methods, with different information transfer 
functions. These include TEM techniques such as phase plate, multi-pass TEM, 
and off-axis holography, as well as STEM techniques, such as ptychography, and 
a quantum sorter.

Vivian Stojanoff, PhD
Education, Training, Outreach
User Program
Center for Biomolecular Structure
National Synchrotron Light Source II
Brookhaven National Laboratory
p 1(631) 344 8375
e [email protected]<mailto:[email protected]>
w https://www.bnl.gov/nsls2/lifesciences/



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