Hi everyone I was working on a protein complex where one protein binds with the other protein majorly based on its surface charge. The protein complex yields nice crystals but does not diffract. Additionally the crystals used to dissolve within the drop when allowed for incubation both at 18 degrees and 4 degrees. Repeated rMMS microseeding, construct variations and changes in purification strategies did not yield any diffractions. Conditions involved for the protein complexes primarily involved PEG with various strengths and pH variations. Looking for your suggestions and valuable ideas
Thanks in advance Dr Rakesh Chatterjee Umea University Umea Sweden ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
