Posted on behalf of Dr. Franck Martin (CNRS IBMC, Strasbourg, France) Please reply to [email protected]
======================================================================================= URGENT - Recovery Plan Project - Post-doctoral fellow(M/F) in molecular and structural biology (Cryo-EM) Mission You will be working asa post-doctoral researcher in a mixed public/private environment. The team'Evolution of translation initiation systems in eukaryotes' of the unit'Architecture and Reactivity of RNA' (UPR 9002 of the CNRS) of the Institute ofMolecular and Cellular Biology (IBMC) of Strasbourgin association with the company Novalix. NovAliX is a 180+ employees societybased in Illkirch (near Strasbourg).We offer to the pharmaceutical industry a complete range of services spanningbiophysics, biochemistry, structural biology, chemistry & drug discovery. Itis specified that this recruitment offer is part of the France RecoveryPlan on a research collaboration project with a company which will be submittedto the DRARI for validation. The project will consist in structurally andfunctionally characterizing macromolecular complexes allowing the initiation ofviral RNA of SARS-CoV-2. Activities The approaches used will be based on the use ofcell-free translation extracts that will allow us to assemble and purifytranslation initiation complexes on our model messenger RNAs. The compositionof these complexes will be determined by mass spectrometry and their structureby advanced structural approaches. Thetechniques used will be: - Biochemical and molecular biology methods dedicatedto the study of proteins and nucleic acids and in particular RNA - Cryo-EM sample preparation, optimizing samplevitrification conditions. - Collect and analyse cryo-EM data. 3D reconstruction,structure determination and atomic model building. - Preparation of in vitro cell-free translationextracts The candidate will have to develop his/her projectindependently. Expectedqualifications and proficiencies This includes the mastery of biochemical and molecularbiology approaches for the purification of ribonucleoprotein complexes. Strongexpertise in structural biology (Crystallography/Cryo-Electron Microscopy)especially on macromolecular complexes containing the ribosome will beconsidered as a plus. Ideally, the candidate should also be able to interpretelectron density maps to establish atomic models of ribonucleic complexescontaining the ribosome. The candidate must have defended his/her thesis in2019 or 2020. Environment The candidate will join a biochemistry and molecularbiology team specialising in translation initiation in eukaryotes. Thestructural part of the project will be implemented within the company Novalix. TheNovalix cryo-EM platform is currently equipped with a Glacios microscopeoutfitted with a phase plate, Falcon 3 camera and microED setup. The candidatewill spend 20% of his/her time in the public laboratory for the preparation ofsamples and 80% of his/her time at Novalix for the structural aspect. More on IBMC: https://ibmc.cnrs.fr/en/ More on Strasbourg:https://www.visitstrasbourg.fr/en/welcome-in-strasbourg/ More on Alsacearea: https://www.visit.alsace/en/ Work constrains and professional risks The project will require the manipulation ofradioactivity and the synthesis of RNA. Therefore, the mastery of good laboratory practices will be considered aplus. Application Applications will only be made via the recruitmentwebsite but feel free ton contact Dr. Franck Martin for any further detailsincluding application rules. Application website: https://emploi.cnrs.fr/Offres/CDD/UPR9002-FRAMAR-006/Default.aspx?lang=EN Salary Gross salary will be between 2675and 3767 € per month according to working experience. Contact information: [email protected] Philippe BENAS, Ph.D. ARN UPR 9002 CNRS IBMC Strasbourg 2, Allée Conrad Roentgen F-67084 STRASBOURG cedex +33.3.8841.7109 E-mails: [email protected], [email protected] URLs: https://ibmc.cnrs.fr/, https://ibmc.cnrs.fr/laboratoire/arn/ ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
