Hi Bulletin members My question is relatively general regarding some protein engineering
The aim is to design a peptide of approx length 25 nm mostly helical in nature that can conduct current. The ends of the peptide have to have some properties that it can bind to metal electrodes (Ruthenium/palladium). The current design is a repeat of EAAAR x 33 units flanked on the N-terminus with a tag (to purify the expressed peptide) and some known gold binding peptide sequences (QQSWPISGSG). A prediction looks like this will give what is desired. Yet, few questions in mind are even the design is to obtain a long helical structure. A voltage applied is expected to produce a dipole and keep the peptide to remain mostly as a strand. These are just assumptions. I want to get this community's valuable suggestions for this design Questions that would like to get some answers are 1) Does anybody have suggestions for what would be the best design to have a peptide that can stick to metal surfaces. 2) what can be done to keep a 25 nm long helical peptide not form any higher-order conformation in solution. 3)Any other ideas people may have here are also welcome Thank you all in advance and hoping to hear from all of you guys. Best regards Padayatti Note: no crystal structures or any scope to do anything like that is not in the scope at the moment -- P -- P -- P ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
