Hi, Try Sigma T8253 - 47 mg/ml, 10 mg/mL benzamidine, 3 mM CaCl2 (uninhibited trypsin tends to chew itself up and not crystallise very well)
Cheers, Janet Janet Newman Principal Scientist / Director, Collaborative Crystallisation Centre (C3) CSIRO Material Science and Engineering 343 Royal Parade Parkville. VIC. 3052 Australia Tel +613 9662 7326 Email [email protected] ________________________________ From: CCP4 bulletin board <[email protected]> on behalf of Murpholino Peligro <[email protected]> Sent: Wednesday, August 25, 2021 2:05 AM To: [email protected] <[email protected]> Subject: [ccp4bb] catalog number for bovine trypsin? Dear all... I want to crystallize trypsin, lots of pdb entries, but so far I have not been able to found the catalog number from which it was crystallized... I see many papers with something like: "Lyophilized bovine pancreas trypsin powder (MW 23 460 Da) was used as purchased (Sigma Chemicals) without any further purification" sometime there is no mention at all if it was purchased or not. If any of you know of a cheap trypsin version (able to crystallize) that would help me a lot. Also any tips to this particular protein would be appreciated... Thanks ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
