Phos-tag gels are an option (albeit a fiddly one). Cheers, Pat Loll
___________________________ Patrick J. Loll, PhD (he, him, his) Professor of Biochemistry & Molecular Biology Drexel University College of Medicine Room 10-102 New College Building 245 N. 15th St. Philadelphia, PA 19102-1192 USA (267) 359-2653 [email protected] > On 15 Sep 2021, at 8:28 AM, Andrea Moretti <[email protected]> wrote: > > Dear CCP4 community, > > sorry for the off topic question but I am struggling with enzymatic assays > and I would need your help. > > I am trying to measure dephosphorylation of a phosphorylated kinase by a > ser/thr phosphatase. So far I tried to measure it by malachite green assay, > anti pSer/pThr antibody and PNPase coupled assay but nothing really worked > for me. NMR is unfortunately not an option since I can't make enough of the > proteins. > > So any hints on phosphatase assays would be helpful. > Thanks! > > Best wishes, > Andrea > > > -- > Andrea Moretti > Structural Plant Biology Laboratory > Department of Botany and Plant Biology > Sciences III > University of Geneva > 30 Quai E. Ansermet > 1211 Geneva > Switzerland > Email: [email protected] > Phone: +41 22 379 3029 (office) > web:http://structplantbio.org > > ######################################################################## > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > > This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing > list hosted by www.jiscmail.ac.uk, terms & conditions are available at > https://www.jiscmail.ac.uk/policyandsecurity/ Patrick Loll [email protected] ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
