The meter doesn't matter so much as the electrode. For the meter, anything with at least 3 pH multipoint calibration is sufficient for most purposes. I usually bought something from our preferred university vendor a with very large LCD displays for my aging eyes.
For protein work, and especially if using anything with Tris buffers, a refillable, double junction Ag/AgCl reference combination electrode is a must. Making stock 1M Tris buffer concentrate will wack out single junction combination electrodes, sometimes permanently. I liked the Fisher Accutuph/Accuphast electrodes because they were much more difficult for my students to break, and they last for years. (My students never succeeded in breaking one.) Gel-filled electrodes are garbage. Refillable double junction electrodes are the way to go for stable readings, especially in Tris, and minimizing heavy metal contamination. Roger Rowlett Gordon & Dorothy Kline Professoe, Emeritus Department of Chemistry Colgate University On Mon, Sep 20, 2021, 4:57 PM Patrick Loll <pjl...@gmail.com> wrote: > Fellow protein biochemists: > > My ~30 year-old Beckman pH meter is finally showing its age, and I’m > looking for a high-quality replacement that doesn’t cost insane amounts of > money. My initial thoughts gravitate toward Mettler (it’s a name I trust, > and some of their low-end instruments aren’t absurdly expensive); would > anybody care to recommend other options? > > Much obliged for any suggestions (the more obsessive, the better). > > Cheers, > > Pat > > > > --------------------------------------------------------------------------------------- > Patrick J. Loll, Ph. D. (he, him, his) > Professor of Biochemistry & Molecular Biology > Drexel University College of Medicine > Room 10-102 New College Building > 245 N. 15th St., Mailstop 497 > Philadelphia, PA 19102 USA > > (215) 762-7706 > pjl...@gmail.com > pj...@drexel.edu > > ######################################################################## > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > > This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a > mailing list hosted by www.jiscmail.ac.uk, terms & conditions are > available at https://www.jiscmail.ac.uk/policyandsecurity/ > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
