Dear Rohit, Do you mean that the residues after your "C-terminus" have been deleted because there is no convincing electron density for them? In that case, a charged carboxylate at the N-terminus is incorrect and you should delete the OXT atom. Alternatively, you could add one residue at the C-terminus and delete all atoms except the N. however, you may still have to manually delete the OXT atom.
Best, Herman Von: CCP4 bulletin board <[email protected]> Im Auftrag von rohit kumar Gesendet: Mittwoch, 22. September 2021 23:29 An: [email protected] Betreff: [ccp4bb] Protein's C-terminal neutral Hello Everyone, Is there any way that I could make my protein's C-terminal neutral using Coot? Actually, I have a protein-peptide complex structure and my peptide is bound at the C-terminal end. While making a surface charge diagram it is negatively charged (because of the CO group at the end, which is not the end residue of my purified protein) and I want to make it neutral (possibly a peptide bond in solution). Please let me know if I am clear enough with my question. Thank you -- Regards Dr. Rohit Kumar Singh Postdoctoral fellow Aurora CO USA ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1<https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
