Dear all, thanks all for the several references you emailed around.
Here's a summary:
A Suite of Engineered GFP Molecules for Oligomeric Scaffolding
https://www.sciencedirect.com/science/article/pii/S0969212615002890
Dimerization properties of the RpBphP2 chromophore-binding domain
crystallized by homologue-directed mutagenesis
https://pubmed.ncbi.nlm.nih.gov/22868772/
An approach to crystallizing proteins by synthetic symmetrization
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1637565/
Why protein crystals favour some space-groups over others
https://www.nature.com/articles/nsb1295-1062
Infinite Assembly of Folded Proteins in Evolution, Disease,and Engineering
https://onlinelibrary.wiley.com/doi/pdf/10.1002/anie.201806092
James Holton duly challenged me, and here's my reply:
JH: If I told you "no, that's not true", how would you go about
proving me wrong? What would that data look like?
--
FvD: Geezus, I don't know! It's not me that hurled this brainfart
around the grant funding agencies 20 years ago... That's why I asked
the Social Brain.
But my primary goal was to have something to stick into the
introduction to a paper, so that has been achieved. Whether it's
ethical to perpetuate a brainfart by citing other expressions of the
brainfart is a reasonable question, but this one would rank quite
low on the impact-of-transgression scale, methinks.
But Dhiraj's reply to my original question is a surely a better one:
/"Recently there were few articles where synthetic symmetrization was
used to enhance the crystallizability. proteins used were MBP, lysozyme
and GFP to name a few."//
/
It implies the weight-of-evidence is out there - hint to you
enterprising students, looking for something *actually useful* to write
a review about!
Frank
On 12/11/2021 14:52, Frank von Delft wrote:
Hello all
Two decades ago, I remember (!) much talk about a reason that
bacterial proteins crystallize "more easily" is that they tend to come
as oligomers (dimers and up), and that this internal symmetry made
them happier to crystallize.
Did anybody ever publish hard evidence? Or even, is there a primary
citation for the idea?
Thanks
Frank
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