We have had success placing single trp on the outside of proteins, as weird as it sounds it works pretty well. If your protein is easy to express you can try a few choices.
If your protein is difficult to express, a fusion with a trp-positive domain or with gfp may be a better option. There are many other options but their application is best considered with more information in hand Artem On Fri, Feb 18, 2022, 8:02 PM Tanner, John J. <tanne...@missouri.edu> wrote: > Dear CCP4BB, > > We are working on a protein that has no Trp residues, which makes > chromatography challenging due to the low absorbance at 280 nm (Abs 0.1% = > 0.104). Does anyone have experience using mutagenesis to increase the Trp > content of proteins? > > Thanks, > > Jack Tanner > > > > -- > > John J. Tanner > > Professor of Biochemistry and Chemistry > > Associate Chair of Biochemistry > > Department of Biochemistry > > University of Missouri > 117 Schweitzer Hall > > 503 S College Avenue > Columbia, MO 65211 > Phone: 573-884-1280 > > Email: tanne...@missouri.edu <tanne...@missouri.edu> > https://cafnrfaculty.missouri.edu/tannerlab/ > > Lab: Schlundt Annex rooms 3,6,9, 203B, 203C > > Office: Schlundt Annex 203A > > > > > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
