Job description Dr. Julien’s laboratory at The Hospital for Sick Children Research Institute is looking for a Research Fellow to join our Structural Biology team. The successful candidate will participate in the characterization of host-pathogen interactions by using a combination of in silico, biochemical, biophysical, structural, and protein engineering techniques. We are looking for a driven and enthusiastic scientist who demonstrates passion and creativity towards the structure-function study of important pathogenic targets and who can thrive in a highly-collaborative setting.
Roles & Responsibilities * Optimize library designs from in silico structural principles leveraging latest developments in structure prediction algorithms; * Design and optimize constructs for expression in E.coli and mammalian cells; * Biophysically characterize purified constructs for their Tm, Tagg and polydipersity, and perform ITC and BLI binding experiments to determine binding thermodynamics and kinetics; * Use Cryo-Electron Microscopy to determine the structure of designed constructs in complex with their receptors to enable iterative structure-based design. Desired Skills and Experience * Structural Biologist with a PhD in Biochemistry, Molecular Biology, Computational Biology or related field; * Strong knowledge in using Cryo-Electron Microscopy to study protein structure, especially in a context of structure-guided design; * Experience in protein engineering, molecular biology, protein expression in various systems, purification and biophysical characterization; * Practical experience in using in silico approaches to improve binding interfaces is a plus; * Demonstrated ability to participate in collaborative scientific projects; * Organization skills, efficiency and meticulous record keeping. How to Apply? Send your CV and a letter of intent to jean-philippe.jul...@sickkids.ca<mailto:jean-philippe.jul...@sickkids.ca> before March 31st, 2022. Successful candidates will be notified for interviews, at which time two letters of reference will also be required. About Us Dr. Julien's laboratory (https://lab.research.sickkids.ca/julien/) focuses on the molecular characterization of biologics by studies of their interactions with cell surface receptors in therapeutic development, and with pathogens guiding vaccine design primarily against Plasmodium falciparum malaria, HIV-1, and COVID-19. Dr. Julien’s laboratory is located in the Molecular Medicine Program of The Peter Gilgan Centre for Research and Learning at SickKids, a 21-story, 750,000 square-foot facility in downtown Toronto that incorporates an open-concept design, state-of-the-art research facilities, and collaborative work spaces. As a member of the SickKids community, you will become part of a world-renowned organization dedicated to fulfilling a vision of Healthier Children, A Better World. For over 135 years, SickKids has worked hard to make the world a better place for children and their families. Each day, people around the organization are doing meaningful work that directly, and often indirectly, benefits children in Canada and around the world. ________________________________ This e-mail may contain confidential, personal and/or health information(information which may be subject to legal restrictions on use, retention and/or disclosure) for the sole use of the intended recipient. Any review or distribution by anyone other than the person for whom it was originally intended is strictly prohibited. If you have received this e-mail in error, please contact the sender and delete all copies. ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
