The PROSS (Protein Repair One-Stop Shop) at: https://pross.weizmann.ac.il/step/pross-terms was used to both express and be able to crystallize human acetylcholinesterase in E. coli, which prior to using PROSS had been impossible.
See paper: Goldenzweig, A., Goldsmith, M., Hill, S. E., Gertman, O., Laurino, P., Ashani, Y., Dym, O., Unger, T., Albeck, S., Prilusky, J., Lieberman, R. L., Aharoni, A., Silman, I., Sussman, J. L., Tawfik, D. S., & Fleishman, S. J. (2016). Automated structure- and sequence-based design of proteins for high bacterial expression and stability. Molecular Cell, 63(2), 337–346. https://doi.org/10.1016/j.molcel.2016.06.012 and Proteopedia's Interactive 3D Complement (I3DC) page: https://proteopedia.org/w/Journal:Molecular_Cell:1 Best regards Joel --------------------------------------------------------------------------------------------- Prof. Joel L. Sussman [email protected]<mailto:[email protected]> Dept. of Chemical and Structural Biology tel: +972 (8) 934 6309 www.weizmann.ac.il/~joel<http://www.weizmann.ac.il/~joel> Weizmann Institute of Science fax: +972 (8) 934 6312 proteopedia.org<http://proteopedia.org> Rehovot 76100 ISRAEL mob: +972 (50) 510 9600 --------------------------------------------------------------------------------------------- On 4 Apr 2022, at 22:06, Scott Classen <[email protected]<mailto:[email protected]>> wrote: Hello CCP4, Has anyone successfully used the available ML/AI protein folding tools to guide crystallization construct design? Maybe you had a protein or domain that was resistant to crystallization efforts and the folding algorithms predicted some loops or termini that were disordered? Then you trimmed or modified them in some way to aid in crystallization? Or if you haven’t done this yourself, are you aware of anyone who has? Thanks, Scott ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Scott Classen, Ph.D. ALS-ENABLE TomAlberTron Beamline 8.3.1 SIBYLS Beamline 12.3.1 Advanced Light Source Lawrence Berkeley National Laboratory 1 Cyclotron Rd MS6R2100 Berkeley, CA 94720 mobile 510.206.4418 desk 510.495.2697 beamline 510.495.2134 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
