Hi, Please check the X and Y of your gain reference file using the e2iminfo.py command. It is possible that those values do not match those of the micrographs. If it is indeed that case, you can use the relion_image_handler options (for example --flipXY) to flip the gain reference file. Hope this helps!
Best, Suparno. On Sat, Jul 16, 2022 at 3:56 PM Jing <zhang...@miamioh.edu> wrote: > Hello All, > I have a question about gain reference file. When I try to work on > Motion Correction on relion 3.1.3, it always reports an error message said > "the size of image and the size of the gain reference do not match." > Because of that error, I could not proceed motion correction of my data. > The cryo EM facility gave me that refence file in dm4 format and I use > dm2mrc to covert it to mrc file. May I ask which part that I did wrong? How > to solve that error? > Thank you > > ######################################################################## > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > > This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a > mailing list hosted by www.jiscmail.ac.uk, terms & conditions are > available at https://www.jiscmail.ac.uk/policyandsecurity/ > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
