On 06/08/2022 09:08, Cryo EM wrote:
Hi everyone,

I am trying to mutate modified RNA nucleotide to standard nucleotide by coot using "Simple Mutate" button. But it is not working despite selecting the correct residue from the standard base list that pop up on clicking "simple mutate button". Does anyone and an idea what couldĀ be the problem? Or is this wrong way to do that?

I am using coot 0.9.8.3 EL (Ccp4) on my Mac.


What does it say in the terminal? - that should give you a clue. My guess is that coot can't auto-superpose onto a non-standard nucleotide.

Try "Replace Residue" instead.

Paul

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