On 07/08/2022 07:00, Cryo EM wrote:
Hi everyone,

I am using Coot 0.9.8.3 EL (ccp4) on my iMac M1 with MacOS Monterey. The phenix version I am using is 1.20.1-4487.

At the end of validation run or real space refinement for my cryo-EM map, I want to fix the problems in the model interactively by the "open in coot" button displayed in phenix. As soon as I click on the problems list in phenix, coot takes me to that atom but I am not able to do any local real space refinement in coot since it complains about the missing restrains for even standard amino acids and nucleotides (screenshot attached).

Please note that coot alone (when not connected to phenix) works for me flawlessly. I guess it has something to do with the missing monomer library path in coot when connected to phenix but I am unable to fix this. Can someone please redirect me to the solution?

Sounds like the monomer library installation is borked.

What does it say in the terminal?

Paul.

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