This is always hard to see this way, but it looks like glycerol at first glance. Try to fit that.
Cheers, Robbie > -----Original Message----- > From: CCP4 bulletin board <[email protected]> On Behalf Of zeyaul > islam > Sent: Tuesday, February 21, 2023 12:13 > To: [email protected] > Subject: [ccp4bb] Unidentified density > > Dear all > > We have solved the structure of nanobody at 1.25 angstrom. We observe some > unidentified density near the serine. Please have a look at the figures. > Protein > was purified in PBS buffer (137 mM NaCl, 2.7 mM KCl, 8 mM Na2HPO4, and 2 > mM KH2PO4) and the crystallisation condition has 0.1 M BIS-TRIS pH 5.5, 2.0 M > ammonium sulfate. Any ideas what this could be? I tried SO4 and PO4 but > didn’t satisfy the observed electron density. > > I appreciate any help you can provide. > > Thanks > > Zeya > > Zeyaul Islam, PhD > > QBRI - Qatar Biomedical Research Institute > > P.O. Box: 34110 > Doha – Qatar > > Tel: +974 445 46690 > > > > ________________________________ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
