Hi Betty,
I've seen very similar looking similar looking density from trifluoroacetic acid that was present with a synthetic peptide. Could be something to check if you have a peptide bound. Best, Alastair ~ Alastair McEwen, PhD Integrated Structural Biology Platform IGBMC 1 rue Laurent Fries 67404 ILLKIRCH - FRANCE tel: +33 (0)3 69 48 52 82 ________________________________ From: CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of Betty Chu <chube...@umd.edu> Sent: 19 April 2023 18:58:53 To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Unknown electron density Dear CCP4BB, I am working on a structure refinement and came across a blob of density that looks like an anvil (see link). Has anyone seen this before and have an idea of what it is? <goog_1600718957> https://drive.google.com/file/d/1BGNwV3MujYjrV4W5m1bh3_WWd49RhJ1w/view?usp=sharing The protein is in potassium phosphate and the components in the crystallization/cryo condition are zinc acetate, PEG, and glycerol. Thank you in advance for your time and help! Betty ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
