We are delighted to announce #time_resolved_23
Find out more and register through https://timeresolved23.le.ac.uk/
*Dynamic Crystallography - XFELs and Synchrotrons to study enzyme reactions
13-15 September, Leicester, UK*
The meeting is sponsored by the UK’s BBSRC Japan Partnering Award Scheme,
the meeting aims to bring researchers from Japan who are developing and
using techniques to exploit XFELs and synchrotrons for time-resolved
studies of enzyme mechanisms together with researchers from the UK and our
partners with similar interests to present and discuss prospects, progress
and, of course, results. All with a view to establishing and furthering
collaborations.
The current planned outline of the meeting/workshop is: (see the meeting
website timeresolved23.le.ac.uk for updates)
*Thursday 14 September *
*Preparation* – How to make suitable samples to enable the study of
transient or short-lived enzyme reaction stats
*Validation* – How to monitor reactions in crystals
*Data Collection* – Strategies and protocols for the collection of
diffraction and spectroscopic data
*Friday 15 September *
*Data Processing* – Strategies, and protocols for the optimal recovery of
high-quality data
*Analysis and interpretation* – How to overcome temporal uncertainty with
respect to reaction coordinates and how to interpret mixed populations.
*Results *– Examples of the application of dynamic investigation of protein
mechanisms using structural and spectroscopic techniques.
At the time of writing we have the following colleagues from Japan who
will participate (with titles of contributions where we have them):
-
- * Hiroshi Sugimoto* (SACLA Spring-8/RIKEN) “Dynamics of hydrogen
atom revealed by ultra-high resolution structure of the heme acquisition
protein”
- *Takehiko Tosha* (University of Hyogo) “Characterization of the
short-lived reaction intermediate of NO reductase with caged substrate”
- *Yoshitsugu Shiro* (University of Hyogo)
- *Hitomi Sawai* (University of Nakasaki)
- *Michihiro Suga* (Okayama University)
- *Minoru Kubo* (University of Hyogo) “Time resolved spectroscopy for
monitoring the protein dynamics in microcrystals”
- *Tetsuo Katayama (Japan Synchrotron Radiation Research
Institute) *"Instrumental
development and data analysis of X-ray emission spectroscopy
supporting SFX
at SACLA"
- * Tetsunari Kimura* (Kobe University) “Development of the
microfluidic mixer for time-resolved SFX and spectroscopy”
Speakers from Europe we currently have confirmed:
- * Allen Orville* (XFEL Hub at Diamond Light Source)
- * Ivo Tews* (University of Southampton)
- * Mike Hough* (Diamond Light Source)*Dr Briony Yorke* (University
of Leeds) "Developing multiplexing methods for time-resolved
crystallographic experiments"
- *Robin Owen* (Diamond Light Source) “Efficient sample delivery for
time resolved SSX and SFX” (TBC)
- *Thomas Ursby *(MAX IV Laboratory) "Serial Crystallography at
MicroMAX - Flexibility offering new possibilities"
- *Emily Freeman* (University of Oxford) “Time-resolved
macromolecular crystallography studies of AmpC from *Escherichia
coli* using
synchrotron and XFEL radiation”.
- *Jack Stubbs* (University of Southampton and Diamond Light Source)
"Size matters: Microfluidics for defining protein crystal dimensions and
rapid micromixing for time-resolved serial crystallography"
- *Patrick Shaw Stewart *(Douglas Instruments) "Preparing crystal
samples for serial data collection and microED: using microseeding and
phase diagrams with microbatch-under-oil"
- *James Beilsten- Edmands* (Diamond Light Source) “Processing serial
diffraction data with DIALS & xia2”.
- *Marcus Gallagher-Jones* (Rosalind Franklin Institute)
- *Thomas Barends* (MPI-IMF Heidelberg)
Please don't use "reply to" with this email, if you want to get in touch
with me use my university email address peter.moody at le.ac.uk
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