Hello, 

I think, in principle this is possible if you use about internal controls. I 
used this approach a long time ago : 

Burmeister WP, Guilligay D, Cusack S, Wadell G, Arnberg N. Crystal structure of 
species D adenovirus fiber knobs and their sialic acid binding sites. J Virol. 
2004 Jul;78(14):7727-36. doi: 10.1128/JVI.78.14.7727-7736.2004. PMID: 15220447; 
PMCID: PMC434083. 

Cheers 
Wim 



De: "Hughes, Jonathan" <jon.hug...@bot3.bio.uni-giessen.de> 
À: "CCP4BB" <CCP4BB@JISCMAIL.AC.UK> 
Envoyé: Mercredi 28 Juin 2023 18:16:22 
Objet: [ccp4bb] quantifying electron density 

hello everyone, 
is there software that can use an electron density map to quantify the number 
of electrons in a selected volume somewhere in a protein? 
cheers 
jon 

-- 
Professor Jon Hughes, BSc, PhD 
Department of Physics 
Free University of Berlin 
Arnimallee 14 
14195 Berlin 
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mobile: (+49/0)1757929098 
email: lv...@posteo.de 
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-- 
Wim Burmeister 
Professor 
Institut de Biologie Structurale (IBS) CIBB 
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