I am afraid most scientists will use the most straightforward technique! If SAD is available the PHOSPHATE backbone of DNA will provide sufficient signal to allow SAD to work, and you get an unambiguous answer to whether it is A-DNA or B or Z... MR will usually work of course as well Eleanor
On Mon, 18 Sept 2023 at 09:18, Natesh Ramanathan <nat...@iisertvm.ac.in> wrote: > Dear Fu Xingke, > > Depends on what Nucleic Acid you are talking of. If it is RNA, > you can expect some sequence to tertiary structure correspondence so you > might be able to try more MR as compared to DNA. DNA may have double > helical architecture but less sequence to tertiary structure > correspondence, and hence DNA is less likely to have a 3D structure like > RNA specific structure for a sequence. > > SAD has become a straight forward method to avoid all these > problems to get ab-initio structure. So many go for it directly. > > Hope that helps. > Best wishes, > Natesh > > On Mon, 18 Sept 2023 at 13:36, fuxingke <fuxingke0...@163.com> wrote: > >> Dear Colleagues, >> Reacently, I find the structures of Nucleic acid are solved by >> single-wavelength anomalous diffraction(SAD). So, why molecular >> replacement (MR) not? >> >> Regards >> >> >> >> Best wishes, >> >> Fu Xingke >> Institute of Physics CAS >> >> ------------------------------ >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 >> > > > -- > ---------------------------------------------------------- > "Live Simply and do Serious Things .. " > - Dorothy Mary Crowfoot Hodgkin OM, FRS > > "In Science truth always wins" > - Max Ferdinand Perutz OM FRS > ---------------------------------------------------------- > Dr. Ramanathan Natesh > Associate Professor, > School of Biology and Center for High-Performance Computing (CHPC), > Founding and Current President of Cryo Electron Microscopy and 3 > Dimensional Image Processing Society of India (CEM3DIPSI), > Indian Institute of Science Education and Research Thiruvananthapuram > (IISER-TVM), > Maruthamala P.O., Vithura, > Thiruvananthapuram, 695551, Kerala, India > > nat...@iisertvm.ac.in > http://faculty.iisertvm.ac.in/natesh > > *Researcher ID*: http://www.researcherid.com/rid/C-4488-2008 > *ORCID*: http://orcid.org/0000-0002-1145-5962 > Vidwan-ID : 94134: http://iisertvm.irins.org/profile/94134 > *PUBLONS*: https://publons.com/author/1520837/ramanathan-natesh#profile > > Office Ph. 0091- 471-2778087 > > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
