These are the ones I've used in the past (I mostly use home-made, since it gives one more control over the process). For folks who prefer convenience, please see below (Thermo's $146/liter, though... LOL). Also, in fairness, at least the Millipore/Sigma offers you isotopically defined media preparations.
https://formedium.com/product-category/formedium-media/escherichia-coli-media/aim-auto-induction-medium/ https://bocascientific.com/gcm20-0500-506-detail Overnight Express (VWR or Millipore) Magic Media (Thermo) I do NOT recommend autoclaving complete formulations (like the Boca Sci. one above) because sugars in the medium react with amino acids resulting in Brown Evil. Look it up if you're curious... In recognition of the original author, references to Studier papers: 2005: https://pubmed.ncbi.nlm.nih.gov/15915565/ 2014: https://pubmed.ncbi.nlm.nih.gov/24203322/ Artem - Cosmic Cats approve of this message On Mon, Oct 2, 2023 at 10:15 AM Artem Evdokimov <[email protected]> wrote: > Dear Jinhui Dong > > You can simply make swimming-pool size amounts of it, as long as you have > basic lab equipment and supplies including an autoclave, and access to ~8 > chemicals. The recipe is in the Studier papers, but I am happy to share it > with you here if you prefer. > > Artem > > - Cosmic Cats approve of this message > > > On Mon, Oct 2, 2023 at 9:39 AM Dong, Jinhui <[email protected]> wrote: > >> Hi, >> Could you please post the following message on ccp4bb? >> >> Autoinduction medium >> We need autoinduction medium for protein expression in E coli. Could >> anyone provide information on USA suppliers with trustworthy products and >> reasonable prices? Thank you! >> Jamse >> Brown University >> >> Thanks! >> >> Jinhui Dong >> >> ------------------------------ >> >> To unsubscribe from the CCP4BB list, click the following link: >> https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 >> > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
