Hello Firdous,
Probably the problem is that mol A and mol B are two different models.  You 
can't add a bond in Chimera or ChimeraX until after combining them into one 
model first.

ChimeraX combine command:
<https://rbvi.ucsf.edu/chimerax/docs/user/commands/combine.html>

ChimeraX bond command or Build Structure tool, Adjust Bonds section:
<https://rbvi.ucsf.edu/chimerax/docs/user/commands/bond.html>
<https://rbvi.ucsf.edu/chimerax/docs/user/tools/buildstructure.html#bonds>

For the best chance of getting an answer to a question about ChimeraX, please 
use the [email protected] <mailto:[email protected]>address:
<https://rbvi.ucsf.edu/chimerax/docs/contact.html>

I hope this helps,

--Eric

        Eric Pettersen
        UCSF Computer Graphics Lab

> From: Collaborative Computational Project in Electron cryo-Microscopy 
> <[email protected] <mailto:[email protected]>> on behalf of Firdous 
> Tarique <[email protected] <mailto:[email protected]>>
> Date: Thursday, November 9, 2023 at 12:27 PM
> To: [email protected] <mailto:[email protected]> <[email protected] 
> <mailto:[email protected]>>
> Subject: [ccpem] Making thioester bond in Chimera/ChimeraX
> 
> Hi
>  
> Does anybody know how to make thioester bonds in Chimera or ChimeraX ?
>  
> I am unable to make a bond between Cys321 in mol A with Gly1 in mol B 
> (S-glycyl-L-cysteine) using Chimera or ChimeraX.
>  
> I tried Isolde in ChimeraX and Build Structure in Chimera to make a bond 
> between selected atoms but so far have been unsuccessful. I don't know if I 
> am missing something.
>  
> Any help would be appreciated.
>  
> Thanks
>  
> Firdous
>  

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