Hi Careina
Initially I was sure they were protein crystals, principally because you only
have NaCl, Hepes and TCEP in your buffer. And then you said they are hard. If
you try to press them against the bottom of the well, they should easily break
if they are made of proteins. Once they did not diffract, I think it is
something you should try with the remaining ones. Alternatively, if you have a
source of UV light coupled to a camera, they should “light up”. I wish you the
best of luck.
Rafael Marques da Silva
PhD Student – Structural Biology
University of Leicester
Mestre em Física Biomolecular
Universidade de São Paulo
Bacharel em Ciências Biológicas
Universidade Federal de São Carlos
phone: +55 16 99766-0021
"A sorte acompanha uma mente bem treinada"
________________________________________________
De: Jurgen Bosch<mailto:[email protected]>
Enviado:quarta-feira, 8 de novembro de 2023 17:08
Para: [email protected]<mailto:[email protected]>
Assunto: Re: [ccp4bb] What could these crystals be?
You could also run them on a gel if you don’t want to shot with canons on small
birds.
Jürgen
On Nov 8, 2023, at 11:33 AM, M T <[email protected]> wrote:
Dear Careina,
If you have easy access to mass spectrometry, you can try to fish/rince your «
pumpkin seeds » and send them to mass to try to identify what is inside to see
if it needs optimization or not.
Best.
Le 8 nov. 2023 à 16:03, [email protected] a écrit :
Hi all
We have been trying with no success to crystalize a protein. Recently we got
these strange shape "crystals". They are hard and flat but they do not diffract
at all. Any ideas as to what could cause this?
Careina
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