Nick,

What files do you mean when call them CCP4 formatted? Coordinates are accepted 
only in mmCIF. Experimental data in mtz. What else?

Thank you.

Vaheh

From: CCP4 bulletin board <[email protected]> On Behalf Of Nicholas Clark
Sent: Wednesday, January 31, 2024 3:49 PM
To: [email protected]
Subject: Re: [ccp4bb] Error in "Prepare and validate files for deposition" task"

Maria,

Apologies for the second email. I forgot that "Prepare files for deposition" 
also merges the CCP4 formatted files into an MTZ. If I recall correctly, I used 
"CONVERT2MTZ" in the command line to complete the merging of the CCP4 files to 
generate the necessary MTZ for deposition. Instructions can be found here: 
https://www.ccp4.ac.uk/html/convert2mtz.html<https://www.ccp4.ac.uk/html/convert2mtz.html>.

Best regards,

Nick Clark

On Tue, Jan 30, 2024 at 12:47 PM mdimarog 
<[email protected]<mailto:[email protected]>> wrote:
Dear all,

I am trying to deposit 2 structures to the PDBe, solved and refined in
CCP4 (version 7), and checked in PRIVATEER as they have a lot of sugars
in. To this end, I used "Prepare and validate files for deposition"
task, as I had previously (and succesfully) done. However, and in spite
of switching to the latest CCP4 version (8.0.017), I keep getting this
error message "adding_stats_to_mmcif_i2_gui:Error in wrapper Failed to
add 204". I am using macOS Monterey, version 12.6.5.

Does anybody have an idea what is going wrong? If not, does anybody know
a different way to submit the reflection data to the PDBe? For example,
would this be somehow possible just using the output from Refmac and
AIMLESS? I wouldn't want to repeat the scaling/MR/refinement procedure
because there are a lot of sugars there, quite difficult to built.

Many thanks in advance for your help!

Kind regards,

Maria




--
Maria Dimarogona
Assistant Professor
Department of Chemical Engineering
University of Patras
Greece

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--
Nicholas D. Clark (He/Him)
PhD Candidate
Malkowski Lab
University at Buffalo
Department of Structural Biology
Jacob's School of Medicine & Biomedical Sciences
955 Main Street, RM 5130
Buffalo, NY 14203

Cell: 716-830-1908

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