Hi Kavya A few things I would try:
-set up your plates (and incubate) at 4 degrees - perhaps in a cold room -use Alphafold model to identify patches of charge that you can mutate -perhaps even introduce a few sticky motifs (e.g. herein and ref 26 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2567453/) -set your drops up at protein:reservoir ratios that aren't 1:1 e.g. 3pro:1res - will eventually equilibrate to greater protein concentration -if super desperate, clone out a small domain, crystallize and then seed back the full-length protein with these Good luck! Andy ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
