Hi Flavio
While i am not aware of any commercial vector with cleavable c
terminal tag, you can easily make your own by introducing protease cleavage
site of your choice. However this strategy will results in quite a few extra
residues from protease site.
An alternative, which is not commercial but available through addgene, is
cpd-his tag. It’s a bigger tag (~25 kda) but for me, it helped in solubility
and expression.
https://www.addgene.org/38251/
There are other variants of this vector available on addgene that you can
choose from. Depending on your c terminal sequence, it may leave either no
extra residues or only one or two residues.
Dhiraj
________________________________
From: CCP4 bulletin board <[email protected]> on behalf of Flavio Di Pisa
<[email protected]>
Sent: Friday, February 16, 2024 3:33 AM
To: [email protected] <[email protected]>
Subject: [External] [ccp4bb] Off topic : plasmid expression vector for E.Coli
with a cleavable his-tag at the c-ter
Dear community,
I'm looking for a commercial expression vector for E.Coli with a
cleavable his-tag at the c-ter. Anyone can help me?
Thank you in advance,
Flavio.
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