Dear all, I'm sure this issue comes up very often, but for the first time in our lab we need to recover a pET-type expression plasmid from a BL21-like E. coli strain (NEB's T7 Express). I know a RecA+ strain is not suitable for plasmid production, but the basic plan is to grow and mini-prep the cells to recover the plasmid and later transform another E coli strain (Top10) to make frozen stocks and for plasmid production. Is this a regular practice or is there any known protocol we should follow? Any advice will be greatly appreciated.
Best wishes, Javier -- Dr. Javier M. González Instituto de Bionanotecnología del NOA (INBIONATEC-CONICET) Universidad Nacional de Santiago del Estero (UNSE) RN9, Km 1125. Villa El Zanjón. (G4206XCP) Santiago del Estero. Argentina Tel: +54-(0385)-4238352 Email <[email protected]> Twitter <https://twitter.com/_biojmg> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
