It’s the Easter Bunny! But seriously, it looks like more than one compound, perhaps hydrogen bonded to each other.
Diana Tomchick Sent from my iPhone On Mar 7, 2024, at 7:32 AM, Eleanor Dodson <0000176a9d5ebad7-dmarc-requ...@jiscmail.ac.uk> wrote: EXTERNAL MAIL Anyone ANY idea what this could be?? Beautiful density but... I am told: The possible/likely components of the protein solution/cryo/crystallisation buffer are: NaCl, MES, a tiny bit of HEPES possibly, ligand ( GR8.027 = the pentasaccharide near catalytic residues Glu276 and Glu165), Et3N which is monomer TEA (a possible counterion for the ligand - see diagram below), glycerol (cryo) There may also be carbonate ions present from the ligand. Any thoughts appreciated!! ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1<https://urldefense.com/v3/__https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1__;!!MznTZTSvDXGV0Co!BTiH5TSnbO7o1AdycsKLUeqlxOH0zDFLZOCaRk44lLnDm50FGyUcbFkqkexX5FyqjoHE62UGpa7PFRnUtPdZ2GV0OMolubR4ZCzDuNETlorgFEc$> CAUTION: This email originated from outside UTSW. Please be cautious of links or attachments, and validate the sender's email address before replying. <coot.png> <coot1.png> ________________________________ UT Southwestern Medical Center The future of medicine, today. ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
