I think you are right, in the final equilibrium all the moisture would condense on the (evaporator?) coils, and there should be provision for them to drip into a reservoir outside. However each time the door opens and humid air is admitted, there will be condensation everywhere. If you leave the plate reader powered on, it will be slightly warmer (some parts more so than others) than the walls, floor, and bench, so will not be able to compete effectively for collecting moisture, and will be the first to dry off as the humidity goes down. The real chance for condensation is when you finish and take it out of the cold. Laptops I enclose in a plastic bag when removing from the cold, don't know how practical that would be for your plate reader. eab
Markus Seeliger wrote on 7/26/2024 11:09 AM:
Dear All, apologies for the off-topic question. I am facing the problem of running enzyme activity assays at low temperature (close to 4C would be ideal) in a fluorescence plate reader. Before I get labeled in our department as "the one who voids warranties" and destroys our beloved plate reader, I wanted to pick your communal brains for any experience on this matter. It is odd - we have had FPLCs, drop setting robots, PCs etc in the cold room for years without much problem, but I have scruples about putting a plate reader in frigid environments. I assume that humidity and condensation would be the number 1 threat to the plate reader (aside from yours truly and his merry coworkers), and if you have any advice on how to minimize humidity in the cold environment, I would appreciate any suggestions. Am I wrong to think that most condensation should occur on the heat exchangers of the cold box/cold room which are the coldest spot in the system and therefore humidity should be reasonably low within the chilled volume (unless we frequently open and close the doors to the jungle-like atmosphere of the lab)? Thank you for your advice Markus ******************************************************* Markus Seeliger Professor Department of Pharmacological Sciences Stony Brook University Medical School, BST 7-170 Stony Brook, NY 11794-8651 office: (631) 444-3558 lab: (631) 638-1299 fax: (631) 444-9749 https://www.pharm.stonybrook.edu/markus-seeliger-lab-welcome [email protected] <mailto:[email protected]> ******************************************************* ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1
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