Hi folks,
I have been trying to prepare a lipid mixture for nanodiscs but I have been
failing miserably to solubilize 1,2-Dimyristoyl-sn-glycero-3-phosphocholine
(DMPC) in my buffer.
What I did so far was preparing 100 mM DMPC in chloroform, dry it out in a
round bottom flask and ressuspend the lipid in 20 mM Tris/HCl pH 7.4, 100 mM
NaCl. 0.5 EDTA and 200 nM Na Cholate. We treated the mixture at hot temperature
(60 to 100 Celsius) and sonicated it for long periods of time with no success.
The protocol descbribed in the literature says the solution shoud be clean, but
this is not what I am getting. I wonder if someone experienced this issue
before and could shed light in how it was solved.
Best wishes
______________________________________________________
Rafael Marques da Silva
PhD Student – Structural Biology
University of Leicester
Mestrando em Física Biomolecular
Universidade de São Paulo
Bacharel em Ciências Biológicas
Universidade Federal de São Carlos
phone: +55 16 99766-0021
"A sorte acompanha uma mente bem treinada"
________________________________________________
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